Part:BBa_K1667002:Design

opdA coding sequence

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal XhoI site found at 1060
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal NgoMIV site found at 157
  Illegal AgeI site found at 352
  Illegal AgeI site found at 691
  
• 1000
  COMPATIBLE WITH RFC[1000]

Design Notes

There is a ompA signal peptide in front of the opdA gene. The ompA piptide could lead the expressed opdA protein out of the host cells.

Source

The sequence of the opdA gene in this part is from BB_K215090, BB_K215091, and BB_K1010008 in igem.